Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics.
James B McGivneyAndrew T CsordasFaye M WalkerElizabeth R BagleyEmily M GruberPeter L MageJose Casas-FinetMargaret A NakamotoMichael EisensteinChristopher J LarkinRobert J StrouseHyongsok Tom SohPublished in: Analytical chemistry (2018)
Biologic drugs are typically manufactured in mammalian host cells, and it is critical from a drug safety and efficacy perspective to detect and remove host cell proteins (HCPs) during production. This is currently achieved with sets of polyclonal antibodies (pAbs), but these suffer from critical shortcomings because their composition is inherently undefined, and they cannot detect nonimmunogenic HCPs. In this work, we report a high-throughput screening and array-based binding characterization strategy that we employed to generate a set of aptamers that overcomes these limitations to achieve sensitive, broad-spectrum detection of HCPs from the widely used Chinese hamster ovary (CHO) cell line. We identified a set of 32 DNA aptamers that achieve better sensitivity than a commercial pAb reagent set and can detect a comparable number of HCPs over a broad range of isoelectric points and sizes. Importantly, these aptamers detect multiple contaminants that are known to be responsible for therapeutic antibody degradation and toxicity in patients. Because HCP aptamer reagents are sequence-defined and chemically synthesized, we believe they may enable safer production of biologic drugs, and this strategy should be broadly applicable for the generation of HCP detection reagents for other cell lines.
Keyphrases
- label free
- nucleic acid
- rheumatoid arthritis
- gold nanoparticles
- end stage renal disease
- sensitive detection
- newly diagnosed
- induced apoptosis
- ejection fraction
- loop mediated isothermal amplification
- drinking water
- prognostic factors
- single cell
- amino acid
- real time pcr
- cell therapy
- peritoneal dialysis
- high resolution
- high throughput
- emergency department
- cell free
- patient reported outcomes
- signaling pathway
- electronic health record
- mesenchymal stem cells
- transcription factor