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Fluorogenic sydnonimine probes for orthogonal labeling.

Wenyuan XuZhuzhou ShaoCheng TangChun ZhangYinghan ChenYong Liang
Published in: Organic & biomolecular chemistry (2022)
A FRET-based fluorescence turn-on probe is designed, which employs a sydnonimine as the linker to match specific fluorophore and quencher pairs and releases the fluorescence after the "click-and-release" reaction. Furthermore, we realized selective fluorescence labeling by exploiting the mutual orthogonality between sydnonimine-DIBAC and tetrazine-1,3-Cp cycloaddition pairs.
Keyphrases
  • single molecule
  • living cells
  • fluorescent probe
  • energy transfer
  • quantum dots
  • small molecule
  • sensitive detection
  • electron transfer