Blueberry extract and its bioactive compounds mitigate oxidative stress and suppress human lung cancer cell (A549) growth by modulating the expression of p53/EGFR/STAT3/IL6-mediated signaling molecules.
Kalaiselvi KrishnamoorthySathan Raj NatarajanVishnu Priya VeeraraghavanSelvaraj JayaramanPublished in: Cell biochemistry and function (2024)
Bioactive phytocompounds are crucial components in all plants. Since the time of traditional medicine, the utilization of plants has been grounded in the potential of these bioactive compounds to treat or manage specific illnesses. These natural bioactive compounds have sparked growing interest in employing medicinal plants for addressing various conditions, such as inflammatory diseases, diabetes, and cancer. This study focuses on assessing the qualitative phytochemical composition, antioxidant potential, and cytotoxic effects of blueberry (Vaccinium sect. Cyanococcus) extract using three different solvents, namely water, ethanol, and methanol. The extract exhibited notable antioxidant activities, as evidenced by DPPH and H 2 O 2 free radical scavenging assays. The cell viability assay also demonstrated cell growth inhibition in A549 cells. Furthermore, nine specific phytocompounds sourced from existing literature were selected for molecular docking studies against CDK6 and, AMPK key protein kinases which enhance the cancer progression. The molecular docking results also revealed favorable binding scores, with a high score of -9.5 kcal/mol in CDK6 protein and a maximum score of AMPK with targets of -8.8 kcal/mol. The selected phytocompounds' pharmacodynamic properties such as ADMET also supported the study. Furthermore, rutin stated that pre-dominantly present in blueberry plants shows a potent cytotoxicity effect in A549 cells. Functional annotations by bioinformatic analysis for rutin also revealed the strong enrichment in the involvement of PI3K/AKT1/STAT, and p53 signaling pathways. Based on this analysis, the identified rutin and other compounds hold a promising anticancer activity. Overall, the comprehensive evaluation of both in vitro and in silico data suggests that the Vaccinium sect. Cyanococcus extract could serve as a valuable source of pharmaceutical agents and may prove effective in future therapeutic applications.
Keyphrases
- papillary thyroid
- molecular docking
- oxidative stress
- induced apoptosis
- lymph node metastasis
- pi k akt
- signaling pathway
- cell cycle arrest
- anti inflammatory
- molecular dynamics simulations
- cell proliferation
- dna damage
- diabetic rats
- type diabetes
- systematic review
- squamous cell carcinoma
- ischemia reperfusion injury
- cell cycle
- cell death
- binding protein
- single cell
- skeletal muscle
- cardiovascular disease
- high throughput
- machine learning
- tyrosine kinase
- risk assessment
- adipose tissue
- protein kinase
- amino acid
- big data
- weight loss
- human health
- dna binding
- epithelial mesenchymal transition
- data analysis
- epidermal growth factor receptor