Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry.

Bo Langhoff HøngeMikkel Steen PetersenRikke OlesenBjarne Kuno MøllerChristian Erikstrup

Published in: PloS one (2017)

Changing the thawing procedure significantly impacted PBMC viability and live cell recovery. Evaluating both viability and live PBMC recovery are necessary to evaluate method performance. Investigation of differential loss of PBMC subtypes and phenotypic changes during thawing and incubation requires further evaluation.

Keyphrases

flow cytometry
endothelial cells
minimally invasive