Play is common among the young of many mammalian species. How that play is exhibited results from a dynamic interplay between genetic framework and experiential influences that, in turn, operate on hard-wired brain systems. One approach towards understanding how genes and environment interact with brain substrates to yield a particular playful phenotype is to take advantage of inbred strains of rats that come with a known genetic identity and assess the effects of varying early social experiences and targeted neurobiological interventions on rats of these strains. This paper primarily summarizes research utilizing the F344 inbred strain, a rat that consistently plays less than most other strains.