Metal-dependent glycosylation in recombinant metallothioneins.
Mario García-RiscoAna GonzálezSara CalatayudFrancisco Javier López-JaramilloVeronika Pedrini-MarthaRicard AlbalatReinhard DallingerJosé M Dominguez-VeraOscar PalaciosMercè CapdevilaPublished in: Chemical communications (Cambridge, England) (2022)
We show for the first time glycosylation of recombinant metallothioneins (MTs) produced in E. coli . Interestingly, our results show that the glycosylation level of the recombinant MTs is inversely proportional to the degree of protein structuration, and reflects their different metal preferences.