A targeted metabolomic procedure for amino acid analysis in different biological specimens by ultra-high-performance liquid chromatography-tandem mass spectrometry.
Mercedes CasadoCristina SierraMarta BatlloriRafael ArtuchAida OrmazabalPublished in: Metabolomics : Official journal of the Metabolomic Society (2018)
We report a modified UPLC-MS/MS procedure for the analysis of 42 amino acids and related compounds in different specimens. The method is fast, sensitive and robust, and it has been validated to be an alternative to the traditional IEC procedure as the routine method used in metabolic laboratories. The method greatly decreases the run time of the analysis while displaying good metrological results.
Keyphrases
- tandem mass spectrometry
- ultra high performance liquid chromatography
- simultaneous determination
- amino acid
- high performance liquid chromatography
- ms ms
- liquid chromatography
- gas chromatography
- high resolution mass spectrometry
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- minimally invasive
- high resolution
- clinical practice
- fine needle aspiration
- ultrasound guided
- data analysis