Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.
Alexey N ButkevichGyuzel Yu MitronovaSven C SidensteinJessica L KlockeDirk KaminDirk N H MeinekeElisa D'EstePhilip-Tobias KraemerJohann G DanzlVladimir N BelovStefan W HellPublished in: Angewandte Chemie (International ed. in English) (2016)
A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.