Specific recognition of ribavirin in animal-derived foods by high performance liquid chromatography combined with magnetic solid-phase extraction based on highly selective Zr-Fe3 O4.

For the first time, a magnetic solid-phase extraction with high-performance liquid chromatography detection method using Zr functionalized Fe3 O4 magnetic material to enrich ribavirin was successfully established. Zr components that modified in Fe3 O4 nanoparticles via a simple one-step hydrothermal method was selected in this work to specifically capture ribavirin by the strong chemical bonding between Zr components of Zr functionalized Fe3 O4 magnetic material and cis-hydroxyl of ribavirin, which was confirmed by pseudo-second-order kinetic model. And Fe3 O4 components were selected in this work to achieve simple operation. Under the optimal experimental conditions, proposed magnetic solid-phase extraction with high-performance liquid chromatography detection method along with Zr functionalized Fe3 O4 magnetic material offered a wide range linearity at 10-200 µg/L with correlation coefficient of 0.9978 with low detection limit of 2.68 µg/L for ribavirin. The relative standard deviations obtained from nine parallel extractions of 100 µg/L ribavirin were 4.41% and revealed good repeatability. This established method was successfully applied to detect real samples including chicken liver, egg, and shrimp with satisfactory recoveries of 74.13-92.9%.