Direct comparison of high-temporal-resolution CINE MRI with Doppler ultrasound for assessment of diastolic dysfunction in mice.
Thomas A RobertsAnthony N PriceLaurence H JacksonValerie TaylorAnna L DavidMark F LythgoeDaniel J StuckeyPublished in: NMR in biomedicine (2017)
Diastolic dysfunction is a sensitive early indicator of heart failure and can provide additional data to conventional measures of systolic function. Transmitral Doppler ultrasound, which measures the one-dimensional flow of blood through the mitral valve, is currently the preferred method for the measurement of diastolic function, but the measurement of the left ventricular volume changes using high-temporal-resolution cinematic magnetic resonance imaging (CINE MRI) is an alternative approach which is emerging as a potentially more robust and user-independent technique. Here, we investigated the performance of high-temporal-resolution CINE MRI and compared it with ultrasound for the detection of diastolic dysfunction in a mouse model of myocardial infarction. An in-house, high-temporal-resolution, retrospectively gated CINE sequence was developed with a temporal resolution of 1 ms. Diastolic function in mice was assessed using a custom-made, open-source reconstruction package. Early (E) and late (A) left ventricular filling phases were easily identifiable, and these measurements were compared directly with high-frequency, pulsed-wave, Doppler ultrasound measurements of mitral valve inflow. A repeatability study established that high-temporal-resolution CINE MRI and Doppler ultrasound showed comparable accuracy when measuring E/A in normal control mice. However, when applied in a mouse model of myocardial infarction, high-temporal-resolution CINE MRI indicated diastolic heart failure (E/A = 0.94 ± 0.11), whereas ultrasound falsely detected normal cardiac function (E/A = 1.21 ± 0.11). The addition of high-temporal-resolution CINE MRI to preclinical imaging studies enhances the library of sequences available to cardiac researchers and potentially identifies diastolic heart failure early in disease progression.
Keyphrases
- left ventricular
- magnetic resonance imaging
- mitral valve
- heart failure
- contrast enhanced
- cardiac resynchronization therapy
- left atrial
- hypertrophic cardiomyopathy
- acute myocardial infarction
- aortic stenosis
- mouse model
- high frequency
- blood pressure
- single molecule
- diffusion weighted imaging
- computed tomography
- mass spectrometry
- oxidative stress
- high resolution
- metabolic syndrome
- type diabetes
- blood flow
- stem cells
- acute coronary syndrome
- atrial fibrillation
- high fat diet induced
- adipose tissue
- machine learning
- deep learning
- electronic health record
- atomic force microscopy
- aortic valve
- percutaneous coronary intervention
- coronary artery disease