Automated multi-sample DNA extraction for genotyping live Xenopus embryos.

Narmadha AllesMatthew GuilleDariusz C Górecki

Published in: Developmental dynamics : an official publication of the American Association of Anatomists (2022)

This method allows rapid genotyping during the early stages of Xenopus development, which enables safe identification of mutants. These can be analyzed at early developmental stages or selected for raising without the need for invasive genotyping later, with resource savings and ethical gains in line with the 3Rs principles.

Keyphrases

high throughput
genome wide
cell free
genetic diversity
circulating tumor
deep learning
dna methylation
single molecule
single cell
gene expression
decision making
quantum dots
loop mediated isothermal amplification