Precise Alkoxyamine Design to Enable Automated Tandem Mass Spectrometry Sequencing of Digital Poly(phosphodiester)s.
Kévin LaunayJean-Arthur AmalianEline LaurentLaurence OswaldAbdelaziz Al OuahabiAlexandre BurelFlorent DufourChristine CarapitoJean-Louis ClémentJean-François LutzLaurence CharlesDidier GigmesPublished in: Angewandte Chemie (International ed. in English) (2020)
A major step towards reliable reading of information coded in the sequence of long poly(phosphodiester)s was previously achieved by introducing an alkoxyamine spacer between information sub-segments. However, MS/MS decoding had to be performed manually to safely identify useful fragments of low abundance compared to side-products from the amide-based alkoxyamine used. Here, alternative alkoxyamines were designed to prevent side-reactions and enable automated MS/MS sequencing. Different styryl-TEMPO spacers were prepared to increase radical delocalization and stiffness of the structure. Their dissociation behavior was investigated by EPR and best results were obtained with spacers containing in-chain benzyl ring, with no side-reaction during synthesis or sequencing. Automated decoding of these polymers was performed using the MS-DECODER software, which interprets fragmentation data recorded for each sub-segment and re-align them in their original order based on location tags.
Keyphrases
- ms ms
- tandem mass spectrometry
- ultra high performance liquid chromatography
- high performance liquid chromatography
- single cell
- high throughput
- deep learning
- machine learning
- liquid chromatography
- mass spectrometry
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- gas chromatography
- health information
- high resolution mass spectrometry
- multiple sclerosis
- electronic health record
- high resolution
- healthcare
- atomic force microscopy
- social media
- microbial community
- high speed
- wastewater treatment