Tuning a pyrazoline-based fluorogenic reagent, 3-naphthyl-1-(4-trifluoromethyl)-5-(4-carboxy phenyl)-2-pyrazoline for sensitive precolumn derivatization of aliphatic alcohols and trace analysis by RP-HPLC with fluorescence detection.
Amal Al-SabahiSaleh Al-BusafiBeena VargheseSalma Al-KindyFakhrEldin O SulimanPublished in: Biomedical chromatography : BMC (2021)
A new member of the pyrazoline family, 3-naphthyl-1-(4-trifluoromethyl)-5-(4-carboxy phenyl)-2-pyrazoline has been evaluated as a precolumn derivatization reagent for the analysis of primary alcohols using HPLC. The simultaneous separation of eight alcohol derivatives (C1 -C8 ) within 15 min was achieved on a reverse-phase C8 column with an isocratic elution mode. The derivatives were detected with fluorescence at an emission wavelength of 470 nm when excited at 360 nm. The identification of the corresponding derivatives was carried out by LC-MS/MS and all showed their characteristic parent peak in negative ion mode. The proposed method was validated using normal analytical tools and was found to be excellent. As a preliminary application, our method was used to determine ethanol concentration in alcohol-containing chocolates and cough syrup.
Keyphrases
- ms ms
- liquid chromatography
- simultaneous determination
- solid phase extraction
- high performance liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- energy transfer
- single molecule
- gas chromatography mass spectrometry
- gas chromatography
- structure activity relationship
- photodynamic therapy
- alcohol consumption
- heavy metals
- label free
- solid state