Gene expression profile of adhesion and extracellular matrix molecules during early stages of skeletal muscle regeneration.
Laura Cristina CeafalanMaria DobreElena MilanesiAndrei M NiculaeEmilia ManoleMihaela GherghiceanuMihail E HinescuPublished in: Journal of cellular and molecular medicine (2020)
Skeletal muscle regeneration implies the coordination of myogenesis with the recruitment of myeloid cells and extracellular matrix (ECM) remodelling. Currently, there are no specific biomarkers to diagnose the severity and prognosis of muscle lesions. In order to investigate the gene expression profile of extracellular matrix and adhesion molecules, as premises of homo- or heterocellular cooperation and milestones for skeletal muscle regeneration, we performed a gene expression analysis for genes involved in cellular cooperation, migration and ECM remodelling in a mouse model of acute crush injury. The results obtained at two early time-points post-injury were compared to a GSE5413 data set from two other trauma models. Third day post-injury, when inflammatory cells invaded, genes associated with cell-matrix interactions and migration were up-regulated. After day 5, as myoblast migration and differentiation started, genes for basement membrane constituents were found down-regulated, whereas genes for ECM molecules, macrophage, myoblast adhesion, and migration receptors were up-regulated. However, the profile and the induction time varied according to the experimental model, with only few genes being constantly up-regulated. Gene up-regulation was higher, delayed and more diverse following more severe trauma. Moreover, one of the most up-regulated genes was periostin, suggestive for severe muscle damage and unfavourable architecture restoration.
Keyphrases
- extracellular matrix
- genome wide identification
- skeletal muscle
- transcription factor
- genome wide
- stem cells
- induced apoptosis
- gene expression
- insulin resistance
- dna methylation
- mouse model
- genome wide analysis
- copy number
- oxidative stress
- cell cycle arrest
- biofilm formation
- adipose tissue
- liver failure
- acute myeloid leukemia
- single cell
- metabolic syndrome
- bone marrow
- signaling pathway
- electronic health record
- endoplasmic reticulum stress
- cell therapy
- mesenchymal stem cells
- immune response
- candida albicans
- pseudomonas aeruginosa
- big data
- hepatitis b virus
- aortic dissection