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A new method for long-read sequencing of animal mitochondrial genomes: application to the identification of equine mitochondrial DNA variants.

Sophie Dhorne-PolletEric BarreyNicolas Pollet
Published in: BMC genomics (2020)
Our method to amplify mtDNA and to sequence it using the nanopore technology is usable for mitochondrial DNA variant analysis. With minor modifications, this approach could easily be applied to other large circular DNA molecules.
Keyphrases
  • mitochondrial dna
  • copy number
  • single molecule
  • genome wide
  • oxidative stress
  • dna methylation
  • circulating tumor
  • single cell
  • cell free
  • circulating tumor cells