Translation is actively regulated during the differentiation of CD8+ effector T cells.
Koichi ArakiMasahiro MoritaAnnelise G BedermanBogumila T KoniecznyHaydn T KissickNahum SonenbergRajesh M ValanparambilPublished in: Nature immunology (2017)
Translation is a critical process in protein synthesis, but translational regulation in antigen-specific T cells in vivo has not been well defined. Here we have characterized the translatome of virus-specific CD8+ effector T cells (Teff cells) during acute infection of mice with lymphocytic choriomeningitis virus (LCMV). Antigen-specific T cells exerted dynamic translational control of gene expression that correlated with cell proliferation and stimulation via the T cell antigen receptor (TCR). The translation of mRNAs that encode translation machinery, including ribosomal proteins, was upregulated during the T cell clonal-expansion phase, followed by inhibition of the translation of those transcripts when the CD8+ Teff cells stopped dividing just before the contraction phase. That translational suppression was more pronounced in terminal effector cells than in memory precursor cells and was regulated by antigenic stimulation and signals from the kinase mTOR. Our studies show that translation of transcripts encoding ribosomal proteins is regulated during the differentiation of CD8+ Teff cells and might have a role in fate 'decisions' involved in the formation of memory cells.
Keyphrases
- induced apoptosis
- cell cycle arrest
- gene expression
- cell proliferation
- signaling pathway
- dna methylation
- oxidative stress
- type diabetes
- dendritic cells
- cell death
- immune response
- transcription factor
- adipose tissue
- metabolic syndrome
- intensive care unit
- working memory
- cell cycle
- liver failure
- drug induced
- acute respiratory distress syndrome
- nk cells
- protein kinase
- extracorporeal membrane oxygenation
- smooth muscle