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Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions.

Naira Caroline Godoy PieriAline Fernanda de SouzaRamon Cesar BotigelliLaís Vicari de Figueiredo PessôaKaiana RecchiaLucas Simões MachadoMayra Hirakawa GlóriaRaquel Vasconcelos Guimarães de CastroDiego Feitosa LealPaulo Fantinato NetoSimone Maria Massami Kitamura MartinsDaniele Dos Santos MartinsFabiana Fernandes BressanAndré Furugen Cesar de Andrade
Published in: Stem cell reviews and reports (2021)
Culture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells.
Keyphrases
  • germ cell
  • stem cells
  • induced pluripotent stem cells
  • gene expression
  • transcription factor
  • cell death
  • endoplasmic reticulum stress
  • cell cycle arrest
  • embryonic stem cells
  • pi k akt