High-Quality Assembly and Comparative Analysis of Actinidia latifolia and A. valvata Mitogenomes.

Kiwifruit ( Actinidia ) has been recently domesticated as a horticultural crop with remarkably economic and nutritional value. In this study, by combining sequence datasets from Oxford Nanopore long-reads and Illumina short-reads, we de novo assembled two mitogenomes of Actinidia latifolia and A. valvata , respectively. The results indicated that the A. latifolia mitogenome has a single, circular, 825,163 bp molecule while the A. valvata mitogenome possesses two distinct circular molecules, 781,709 and 301,558 bp, respectively. We characterized the genome structure, repeated sequences, DNA transfers, and dN/dS selections. The phylogenetic analyses showed that A. valvata and A. arguta , or A. latifolia and A. eriantha , were clustered together, respectively. This study provides valuable sequence resources for evolutionary study and molecular breeding in kiwifruit.