Synergistic activity of diclofenac sodium with oxacillin against planktonic cells and biofilm of methicillin-resistant Staphylococcus aureus strains.
Helaine A QueirozCecília R da SilvaJoão B de Andrade NetoLívia Gurgel do Amaral Valente SáFrancisca Bsa do NascimentoLeticia Ss MorenoFátima Dd BarrosoLisandra J da SilvaThiago M CândidoLeilson C de OliveiraJacó Rl de MesquitaManoel O de MoraesBruno C CavalcantiHélio V Nobre JúniorPublished in: Future microbiology (2021)
Aim: To evaluate the activity of diclofenac sodium and synergism with oxacillin against clinical strains of SARM in plactonic cells, antibiofilm and biofilm. Materials & methods: Synergism activity was assessed using the fractional inhibitory concentration index and its possible mechanism of action by flow cytometry. Results: The synergistic activity of diclofenac sodium with oxacillin was observed against plactonic cells, antibiofilm and in biofilm formed from clinical methicillin-resistant Staphylococcus aureus strains. Conclusion: This combination caused damage to the integrity of the membrane and ruptures in the DNA of the cells, leading to apoptosis.
Keyphrases
- cell cycle arrest
- induced apoptosis
- methicillin resistant staphylococcus aureus
- staphylococcus aureus
- pseudomonas aeruginosa
- cell death
- endoplasmic reticulum stress
- oxidative stress
- escherichia coli
- flow cytometry
- candida albicans
- signaling pathway
- cystic fibrosis
- biofilm formation
- cancer therapy
- cell free
- circulating tumor