Secondary Metabolites with Nitric Oxide Inhibition from Marine-Derived Fungus Alternaria sp. 5102.
Sen-Hua ChenYanlian DengChong YanZhenger WuHeng GuoLan LiuHongju LiuPublished in: Marine drugs (2020)
Two new benzofurans, alternabenzofurans A and B (1 and 2) and two new sesquiterpenoids, alternaterpenoids A and B (3 and 4), along with 18 known polyketides (5-22), were isolated from the marine-derived fungus Alternaria sp. 5102. Their structures were elucidated on the basis of extensive spectroscopic analyses (1D and 2D NMR, HR-ESIMS, and ECD) and X-ray crystallography, as well as the modified Mosher's method. Compounds 2, 3, 5, 7, 9-18, and 20-22 exhibited potent anti-inflammatory activity by inhibiting the production of NO in RAW264.7 cells activated by lipopolysaccharide with IC50 values in the range from 1.3 to 41.1 μM. Structure-activity relationships of the secondary metabolites were discussed.
Keyphrases
- high resolution
- nitric oxide
- ms ms
- induced apoptosis
- signaling pathway
- magnetic resonance
- cell cycle arrest
- molecular docking
- inflammatory response
- magnetic resonance imaging
- hydrogen peroxide
- endoplasmic reticulum stress
- nitric oxide synthase
- oxidative stress
- computed tomography
- solid state
- anti inflammatory
- mass spectrometry
- pi k akt
- cell proliferation
- immune response
- electron microscopy
- contrast enhanced