Design of oriented mesoporous silica films for guiding protein adsorption states.

The highly-oriented cylindrical mesoporous silica films were synthesized on the rubbing-treated polyimide by adjusting the molar ratio of the orientation-directing agent (Brij56) to the structure-directing agent (P123) as surfactants in the silica precursor solutions for guiding protein adsorption states. As a result, the diameter and the orientation degree of mesopores changed with the molar ratio of Brij56 to P123. The maximum orientation degree (93%) of cylindrical mesopores oriented in the direction perpendicular to the rubbing direction was observed when the molar ratio of Brij56 to P123 was 3. Then, the dissolution features in simulated body fluid and the protein adsorption properties of the oriented cylindrical mesoporous silica films were investigated. The silica skeletons were gradually dissolved from the upper film surfaces and subsequently, the mesopore structures were collapsed when the films were immersed for 90 min. Moreover, the protein adsorption amount and the ratio from the mono-component and two-component solutions on the films were higher than those on the unoriented cylindrical mesoporous silica films due to the formation of open-ended cylindrical mesopore shapes and sizes. In addition, the shapes of the proteins adsorbed on the films had anisotropy, which would be reflected by the cylindrical mesopore shapes generated by the dissolution of silica layers and subsequent exposure of inner mesopore surfaces. Therefore, the synthesized highly-oriented cylindrical mesoporous silica films were useful to adsorb mesoscale biomolecules such as proteins and can effectively guide their anisotropic adsorption shapes, and therefore have the potential to be used as surface-coating films of polyimide in biomedical fields.