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Validation of a rapid potency assay for cord blood stem cells using phospho flow cytometry: The IL-3-pSTAT5 assay.

Carl SimardDiane FournierPatrick Trépanier
Published in: International journal of laboratory hematology (2022)
This study shows that our rapid flow cytometry assay can be successfully validated and that the potency data obtained display good sensitivity, specificity and robustness. These results demonstrate the feasibility of implementing this assay within CBB operations, as a validated potency assay.
Keyphrases
  • flow cytometry
  • high throughput
  • stem cells
  • cord blood
  • mesenchymal stem cells
  • bone marrow
  • big data
  • cell therapy