Photo-induced telomeric DNA damage in human cancer cells.
Justin WeynandHarikleia EpiskopouGabriel Le BerreMartin GillardJérôme DejeuAnabelle DecottigniesEric DefrancqBenjamin EliasPublished in: RSC chemical biology (2022)
Herein we report on the study of novel dinuclear ruthenium(ii) complexes designed to target and to photo-react with G-quadruplex telomeric DNA. Upon irradiation, complexes efficiently generate guanine radical cation sites as photo-oxidation products. The compounds also display efficient cell penetration with localization to the nucleus and show strong photocytotoxicity toward osteosarcoma cells. Thanks to a microscopic-based telomere dysfunction assay, which allows the direct visualization of DNA damage in cells, we brought the first evidence of forming photo-oxidative damage at telomeres in cellulo . This emphasizes interesting prospects for the development of future cancer phototherapies.
Keyphrases
- dna damage
- induced apoptosis
- oxidative stress
- cell cycle arrest
- electron transfer
- endothelial cells
- dna repair
- current status
- dna damage response
- endoplasmic reticulum stress
- diabetic rats
- single cell
- cell therapy
- stem cells
- high glucose
- high throughput
- signaling pathway
- radiation therapy
- ionic liquid
- mesenchymal stem cells
- circulating tumor
- bone marrow
- young adults
- nucleic acid