Paper integrated microfluidic contact lens for colorimetric glucose detection.
Pelin Kubra IsgorTaher AbbasiaslRitu DasEmin IstifUmut Can YenerLevent BekerPublished in: Sensors & diagnostics (2024)
Contact lenses offer a simple, cost-effective, and non-invasive method for in situ real-time analysis of various biomarkers. Electro-chemical sensors are integrated into contact lenses for analysis of various biomarkers. However, they suffer from rigid electronic components and connections, leading to eye irritation and biomarker concentration deviation. Here, a flexible and microfluidic integrated paper-based contact lens for colorimetric analysis of glucose was implemented. Facilitating a three-dimensional (3D) printer for lens fabrication eliminates cumbersome cleanroom processes and provides a simple, batch compatible process. Due to the capillary force of the filter paper, the sample was routed to detection chambers inside microchannels, and it allowed further colorimetric detection. The paper-embedded microfluidic contact lens successfully detects glucose down to 2 mM within ∼10 s. The small dimension of the microfluidic system enables detection of glucose levels as low as 5 μl. The results show the potential of the presented approach to analyze glucose concentration in a rapid manner. It is demonstrated that the fabricated contact lens can successfully detect glucose levels of diabetic patients.
Keyphrases
- label free
- loop mediated isothermal amplification
- blood glucose
- gold nanoparticles
- cataract surgery
- circulating tumor cells
- sensitive detection
- single cell
- hydrogen peroxide
- real time pcr
- fluorescent probe
- type diabetes
- living cells
- high resolution
- adipose tissue
- metabolic syndrome
- quantum dots
- mass spectrometry
- high speed
- insulin resistance