Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein.

Wouter DanielsJeroen BouvinTobias BuscheChristian RückertKenneth SimoensSpyridoula KaramanouLieve Van MellaertÓlafur H FriðjónssonBart NicolaiAnastassios EconomouJörn KalinowskiJozef AnnéKristel Bernaerts

Published in: Microbial cell factories (2018)

Transcriptomic and fluxomic changes uncover potential new leads for targeted strain improvement strategies which may ease the secretion stress and metabolic burden associated with heterologous protein synthesis and secretion, and may help create a more consistently performing S. lividans strain. Yet, links to secondary metabolism and redox balancing should be further investigated to fully understand the S. lividans metabolome under heterologous protein production.

Keyphrases

single cell
saccharomyces cerevisiae
protein protein
rna seq
amino acid
binding protein
small molecule
risk factors
mass spectrometry
risk assessment
stress induced
heat stress