H2A monoubiquitination in Arabidopsis thaliana is generally independent of LHP1 and PRC2 activity.

Our findings show the relationship between H2AK121ub and H3K27me3 marks across the A. thaliana genome and unveil that ubiquitination by PRC1 is largely independent of PRC2 activity in plants, while the inverse is true for H3K27 trimethylation.