Central nervous system mycobacteriosis caused by Mycobacterium genavense in degus ( Octodon degus ).
Tatshuhito IiJames K ChambersMizuho UneyamaAyumi SumiYutaro NakayamaNanae TsuritaYasutsugu MiwaKazuyuki UchidaPublished in: Veterinary pathology (2023)
Degus ( Octodon degus ) that were kept at a breeding facility presented with neurological or respiratory symptoms and died. Necropsies were performed on 9 individuals, and no significant gross lesions were found. Histologically, spinal cord necrosis was observed in all 9 cases and granulomatous myelitis in 5 of the 9 cases. Locally extensive necrosis of the brain and encephalitis were observed in 7 of the 9 cases. Acid-fast bacteria were found in the spinal cords, brains, and lungs from all 9 cases. Immunohistochemically, Mycobacterium tuberculosis antigen was observed in the spinal cords, brains, and lungs from all 9 cases. Double-labeling immunofluorescence revealed M. tuberculosis antigen in IBA1- and myeloperoxidase-immunopositive cells. Extracted genomic DNA from 8 of the 9 cases was successfully amplified with the primers for Mycobacterium genavense ITS1 and hypothetical 21 kDa protein genes, and the polymerase chain reaction products were identified as M. genavense by DNA sequencing. This report highlights the susceptibility of degus to M. genavense infection in the central nervous system.
Keyphrases
- mycobacterium tuberculosis
- spinal cord
- single cell
- oxidative stress
- induced apoptosis
- single molecule
- gene expression
- cerebrospinal fluid
- circulating tumor
- blood brain barrier
- hiv infected
- dna methylation
- endoplasmic reticulum stress
- brain injury
- heat shock protein
- functional connectivity
- resting state
- human immunodeficiency virus
- circulating tumor cells
- cell death