Adaptive optics improves multiphoton super-resolution imaging.
Wei ZhengYicong WuPeter WinterRobert FischerDamian Dalle NogareAmy HongChad McCormickRyan ChristensenWilliam P DempseyDon B ArnoldJoshua ZimmerbergAjay ChitnisJames SellersClare WatermanHari ShroffPublished in: Nature methods (2017)
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface.
Keyphrases
- high resolution
- induced apoptosis
- photodynamic therapy
- single molecule
- high speed
- cell cycle arrest
- white matter
- resting state
- high throughput
- mass spectrometry
- functional connectivity
- cell death
- signaling pathway
- copy number
- optical coherence tomography
- aedes aegypti
- cell proliferation
- cerebral ischemia
- label free
- drosophila melanogaster
- endoscopic submucosal dissection
- zika virus
- single cell