Molecular karyotypes of loquat (Eriobotrya japonica) aneuploids can be detected by using SSR markers combined with quantitative PCR irrespective of heterozygosity.

Guo Wen Jiangbo Dang Zhongyi XieJinying WangPengfei JiangQigao GuoGuolu Liang

Published in: Plant methods (2020)

SSR-qPCR can detect loquat aneuploids and be used to construct the entire molecular karyotypes of aneuploid individuals. Therefore, this method offers a novel alternative for the detection of chromosome aneuploidies.

Keyphrases

real time pcr
genetic diversity
high resolution
single molecule
copy number
mass spectrometry