Aqueous ethanol extract of Libidibia ferrea (Mart. Ex Tul) L.P. Queiroz (juca) exhibits antioxidant and migration-inhibiting activity in human gastric adenocarcinoma (ACP02) cells.
Luana França Calandrini de AzevedoTássia Alana Alves FerreiraKarina Motta MeloClara Louise Porfírio DiasCarlos Eduardo Matos Carvalho BastosSeidel Ferreira SantosAlberdan da Silva SantosCleusa Yoshiko NagamachiJúlio César PieczarkaPublished in: PloS one (2020)
Libidibia ferrea (juca) is a plant belonging to the Fabaceae (Leguminosae) family, whose antioxidant activity has been widely described in the literature. We evaluated this parameter of Aqueous ethanol extract (AE), ethyl acetate (ACO), chloroform (CLO) and hexane (HEX) extracts of L. ferrea. We then tested the most active extract for its toxicity and ability to inhibit migratory activity in the ACP02 gastric adenocarcinoma cell line in vitro. The AE and ACO extracts both had antioxidant activity, the AE extract showing greater potential. This may reflect that both extracts contained phenolic compounds. Although AE extract showed no cytotoxic, mutagenic or genotoxic effect, it altered cell morphology and migration activity. Analysis of apoptosis/necrosis indicated that this parameter does not appear to account for the apparent ability of AE to inhibit cancer cell migration. We speculate that the morphological changes in AE-treated cells could be due to cytoskeleton alterations related to the presence of myo-inositol in AE extract. Together, our results demonstrate this extract of L. ferrea can act as an exogenous antioxidant and might prove useful in efforts to fight secondary tumors.
Keyphrases
- oxidative stress
- induced apoptosis
- anti inflammatory
- cell cycle arrest
- squamous cell carcinoma
- ionic liquid
- systematic review
- endoplasmic reticulum stress
- magnetic resonance imaging
- endothelial cells
- cell proliferation
- signaling pathway
- young adults
- cell therapy
- single cell
- induced pluripotent stem cells
- newly diagnosed