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An improved imaging system that corrects MS2-induced RNA destabilization.

Weihan LiAnna MaekiniemiHanae SatoChristof OsmanRobert H Singer
Published in: Nature methods (2022)
The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.
Keyphrases
  • mass spectrometry
  • multiple sclerosis
  • ms ms
  • living cells
  • high resolution
  • fluorescent probe
  • binding protein
  • signaling pathway
  • single molecule
  • amino acid
  • nucleic acid