Expression of TNF, IL1B, and NOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis.
Endang Winiati BachtiarCitra F PutriRetno D SoejoedonoBoy Muchlis BachtiarPublished in: F1000Research (2020)
Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, NOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and NOS2 of neuron cells.
Keyphrases
- induced apoptosis
- cell cycle arrest
- gene expression
- rheumatoid arthritis
- escherichia coli
- cell death
- dna methylation
- oxidative stress
- pseudomonas aeruginosa
- spinal cord injury
- stem cells
- staphylococcus aureus
- signaling pathway
- genome wide
- immune response
- nitric oxide
- inflammatory response
- white matter
- pi k akt
- transcription factor
- single cell
- resting state
- brain injury
- blood brain barrier
- biofilm formation
- cell therapy