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Development of a Metaphyseal Non-Union Model in the Osteoporotic Rat Femur.

Amelie DelucaAndrea WagnerBettina FaustiniNadja WeissenbacherChristian DeiningerFlorian WichlasHerbert TempferErnst J MuellerAndreas Traweger
Published in: Bioengineering (Basel, Switzerland) (2023)
The aim of this current study was to establish a metaphyseal femoral non-union model in osteoporotic rats by comparing a power tool versus a manual tool for fracture creation. Twelve adult female Sprague Dawley rats were ovariectomized (OVX) and received a special diet for 6 weeks. Biweekly pQCT measurements confirmed a significant reduction in the cancellous and total bone mineral density in OVX rats compared to control (CTRL) animals. After 6 weeks, OVX rats underwent surgery creating a distal metaphyseal osteotomy, either using a piezoelectric- (n = 6) or a manual Gigli wire (n = 6) saw. Fractures were stabilized with a Y-shaped mini-locking plate. Within each group, three rats received Alginate directly into the fracture gap. OVX animals gained more weight over 8 weeks compared to CTRL animals. pQCT analysis showed a significant difference in the volumetric cancellous bone mineral density between OVX and CTRL rats. A histological examination of the osteoporotic phenotype was completed. Radiographic evaluation and Masson-Goldner trichrome staining with the piezoelectric saw failed to demonstrate bony bridging or a callus formation. New bone formation and complete healing were seen after 6 weeks in the Gigli group. For the creation of a metaphyseal atrophic non-union in the osteoporotic bone, a piezoelectric saw should be used.
Keyphrases
  • bone mineral density
  • postmenopausal women
  • body composition
  • minimally invasive
  • gestational age
  • physical activity
  • weight loss
  • acute coronary syndrome
  • total knee arthroplasty
  • young adults
  • bone loss