A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578.

Yi ShenHod DanaAhmed S AbdelfattahRonak PatelJamien SheaRosana S MolinaBijal RawalVladimir RancicYu-Fen ChangLanshi WuYingche ChenYong QianMatthew D WiensNathan HambletonKlaus BallanyiThomas E HughesMikhail DrobizhevDouglas S KimMinoru KoyamaEric R SchreiterRobert E Campbell

Published in: BMC biology (2018)

K-GECO1 is the archetype of a new lineage of GECIs based on the RFP eqFP578 scaffold. It offers high sensitivity and fast kinetics, similar or better than those of current state-of-the-art indicators, with diminished lysosomal accumulation and minimal blue-light photoactivation. Further refinements of the K-GECO1 lineage could lead to further improved variants with overall performance that exceeds that of the most highly optimized red GECIs.

Keyphrases

single cell
quantum dots
cell fate
living cells
protein protein
amino acid
gene expression
binding protein
protein kinase
label free