CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coli.

Qingyang LiPeng ZhaoHang YinZhaonan LiuHaifeng ZhaoPingfang Tian

Published in: Microbial cell factories (2020)

This work demonstrates that CRISPRi system can improve aconitic acid production by coordinating glycolysis and TCA cycle. This study provides insights for high-level production of the intermediate metabolites in central pathways.

Keyphrases

escherichia coli
ms ms
genome wide
type diabetes
blood glucose
adipose tissue
metabolic syndrome
skeletal muscle
insulin resistance