Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA.

Yacine AmarIlias LagkouvardosRafaela L SilvaOluwaseun Ayodeji IsholaBärbel U FoeselSusanne KublikAnne SchölerSebastian NiedermeierRachela BleuelAlexander ZinkKlaus NeuhausMichael SchloterTilo BiedermannMartin Köberle

Published in: Microbiome (2021)

The BDA approach enables both a better assessment of the living microbiota and depletion of host DNA reads. Video abstract.

Keyphrases

circulating tumor
cell free
single molecule
nucleic acid
soft tissue
wound healing
neural network