Microfluidic Chip-assisted Upconversion Luminescence Biosensing Platform for Point-of-care Virus Diagnostics.

Epidemics caused by multiple viruses continue to emerge, which have brought a terrible impact on human society. Identification of viral infections with high sensitivity and portability is of significant importance for the screening and management of diseases caused by viruses. Herein, a microfluidic chip (MFC)-assisted upconversion luminescence biosensing platform has been designed and fabricated for point-of-care virus detection. Upconversion nanoparticles with excellent stability are successfully synthesized as luminescent agents for optical signal generation in the portable virus diagnostic platform. The relevant investigation results illustrate that the MFC-assisted virus diagnostic platform possessed outstanding performance such as good integration, high sensitivity (1.12 pg/ml), ease of use, and portability. In addition, clinical sample test result verifies its more prominent virus diagnostic property than commercially available rapid test strips. All of these thrilling capabilities imply that our designed portable virus diagnostic platform has great potential for future virus detection applications. This article is protected by copyright. All rights reserved.