Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives.
Jea Sung RyuSan Hae ImYoo Kyung KangYang Soo KimHyun Jung ChungPublished in: Biomaterials science (2020)
Gram-negative bacteria are a significant cause of infections acquired in both hospital and community settings, resulting in a high mortality rate worldwide. Currently, a Gram-negative infection is diagnosed by symptom evaluation and is treated with empiric antibiotics which target both Gram-negative and Gram-positive bacteria. A rapid and simple diagnostic method would enable immediate and targeted treatment, while dramatically reducing antibiotic overuse. Herein, we introduce a method utilizing a fluorescent derivative of colistin (COL-FL), that can directly label the Gram-negative cell wall of live bacteria and universally detect the targets within 10 min. By using the COL-FL assay, we achieved the differential labeling of various Gram-negative pathogens related to hospital-acquired infections, which could be subsequently detected via spectrofluorometry and microscopy. Further, we determined that our method can be used for complex samples, such as combinations of multiple bacterial types; bacteria in the presence of mammalian cells; and bacteria with serum components. This assay can be integrated into a simple diagnostic platform for rapid screening tests and the stratification of Gram-negative bacterial infections in the clinic.
Keyphrases
- gram negative
- multidrug resistant
- high throughput
- loop mediated isothermal amplification
- cell wall
- healthcare
- drug resistant
- acinetobacter baumannii
- high resolution
- label free
- primary care
- cardiovascular disease
- mental health
- escherichia coli
- single molecule
- cardiovascular events
- quantum dots
- type diabetes
- risk factors
- acute care
- optical coherence tomography
- living cells
- cystic fibrosis
- replacement therapy
- drug induced