Development and evaluation of a rapid one-step high sensitivity real-time quantitative PCR system for minor BCR-ABL (e1a2) test in Philadelphia-positive acute lymphoblastic leukemia (Ph+ ALL).
Michihiro HidakaKoiti InokuchiNobuhiko UoshimaNaoto TakahashiNao YoshidaShuichi OtaHirohisa NakamaeHiromi IwasakiKenichiro WatanabeYoshiyuki KosakaNorio KomatsuKuniaki MeguroYuho NajimaTetsuya EtoTakeshi KondoShinya KimuraChikashi YoshidaYuichi IshikawaMasashi SawaTomoko HataKeizo HoribeHiroatsu IidaTakeshi ShimomuraNobuaki DobashiIsamu SugiuraJunya MakiyamaNaoyuki MiyagawaAsuka SatoRyuta ItoItaru MatsumuraYuzuru KanakuraTomoki NaoePublished in: Japanese journal of clinical oncology (2023)
The 'Otsuka" minor BCR-ABL messenger ribonucleic acid assay kit exhibits excellent performance in quantifying minor BCR-ABL transcripts in Philadelphia chromosome-positive acute lymphoblastic leukemia patients. Our results align well with established screening methods and show a strong correlation with laboratory-developed tests and diagnostic research products. The "Otsuka" kit holds great promise as a valuable tool for understanding Philadelphia chromosome-positive acute lymphoblastic leukemia pathology and guiding effective treatment strategies.
Keyphrases
- acute lymphoblastic leukemia
- chronic myeloid leukemia
- tyrosine kinase
- allogeneic hematopoietic stem cell transplantation
- end stage renal disease
- ejection fraction
- chronic kidney disease
- newly diagnosed
- peritoneal dialysis
- copy number
- prognostic factors
- high throughput
- gene expression
- big data
- acute myeloid leukemia
- mass spectrometry
- quantum dots