Determination of novel reference genes for improving gene expression data normalization in selected canine reproductive tissues - a multistudy analysis.

Marta NowakSelim AslanMariusz Pawel Kowalewski

Published in: BMC veterinary research (2020)

Based on our analyses, we recommend applying KDM4A and PTK2 for normalization of gene expression in the canine CL and placenta. The inclusion of a third reference gene, EIF4H, is suggested for healthy uteri. With this, the interpretation of qPCR data will be more reliable, allowing better understanding of canine reproductive physiology.

Keyphrases

gene expression
dna methylation
genome wide
electronic health record
big data
genome wide identification
copy number
data analysis
high resolution
solid phase extraction
bioinformatics analysis