Aptamer-AuNP-conjugated carboxymethyl chitosan-functionalized graphene oxide for colorimetric identification of Salmonella typhimurium.
Pian WuRuixue HuangCuimei ChenJiecan YiTianhan KaiYujuan ZhanXiaoqian WeiDanqi WangJingwen ZhangPing DingPublished in: Mikrochimica acta (2022)
A novel aptamer-AuNP-conjugated carboxymethyl chitosan-functionalized graphene oxide (CMC/GO@Apt-Au NP) probe was for the first time developed for the determination of Salmonella typhimurium (S. typhimurium). Owing to the conformational change of the aptamers in the presence of S. typhimurium, the Au NPs, which were pre-adsorbed on the aptamers through van der Waals forces, were released into the solution phase and induced the color change of the solution. As a result, S. typhimurium ranging from 10 2 to 10 7 CFU/mL was successfully identified using the designed assay with a limit of detection (LOD) of 10 CFU/mL. This low detection level allowed the sensitive recognition of S. typhimurium in milk samples within 40 min without sample pretreatment, a conclusion that agreed well with the traditional plate counting method. The developed method not only provides a rapid way for the determination of S. typhimurium with simplicity and sensitivity but also shows potential universality in the quantification of other pathogenic microorganisms.
Keyphrases
- listeria monocytogenes
- sensitive detection
- loop mediated isothermal amplification
- gold nanoparticles
- quantum dots
- drug delivery
- label free
- escherichia coli
- molecularly imprinted
- molecular dynamics
- nitric oxide
- high throughput
- risk assessment
- mass spectrometry
- solid phase extraction
- real time pcr
- living cells
- high glucose
- nucleic acid
- diabetic rats
- drug induced
- oxidative stress
- fluorescent probe
- simultaneous determination