Quick Transformation with Plasmid DNA.
Nara Figueroa-BossiRoberto BalbontínLionello BossiPublished in: Cold Spring Harbor protocols (2022)
Genomic engineering of <i>Escherichia coli</i> and <i>Salmonella</i> often requires introducing plasmids into strains obtained during the intermediate stages of the process. Such strains are typically transformed only once, making the preparation of large batches of competent cells for storage purposes unnecessary. Here, we describe a simple scaled-down procedure for transforming <i>E. coli</i> or <i>Salmonella</i> with plasmid DNA that uses as little as 2 mL of culture.
Keyphrases
- escherichia coli
- circulating tumor
- cell free
- induced apoptosis
- single molecule
- klebsiella pneumoniae
- biofilm formation
- cell cycle arrest
- nucleic acid
- endoplasmic reticulum stress
- gene expression
- circulating tumor cells
- oxidative stress
- signaling pathway
- staphylococcus aureus
- cell proliferation
- multidrug resistant
- pi k akt
- simultaneous determination