Visualizing human photoreceptor and retinal pigment epithelium cell mosaics in a single volume scan over an extended field of view with adaptive optics optical coherence tomography.
Muhammad Faizan ShiraziElisabeth BrunnerMarie LaslandesAndreas PollreiszChristoph K HitzenbergerMichael PircherPublished in: Biomedical optics express (2020)
Using adaptive optics optical coherence tomography, human photoreceptors and retinal pigment epithelium (RPE) cells are typically visualized on a small field of view of ∼1° to 2°. In addition, volume averaging is required for visualizing the RPE cell mosaic. To increase the imaging area, we introduce a lens based spectral domain AO-OCT system that shows low aberrations within an extended imaging area of 4°×4° while maintaining a high (theoretical) transverse resolution (at >7 mm pupil diameter) in the order of 2 µm. A new concept for wavefront sensing is introduced that uses light mainly originating from the RPE layer and yields images of the RPE cell mosaic in a single volume acquisition. The capability of the instrument for in vivo imaging is demonstrated by visualizing various cell structures within the posterior retinal layers over an extended field of view.
Keyphrases
- optical coherence tomography
- single cell
- high resolution
- cell therapy
- endothelial cells
- diabetic retinopathy
- optic nerve
- computed tomography
- magnetic resonance imaging
- induced apoptosis
- machine learning
- stem cells
- gene expression
- deep learning
- mesenchymal stem cells
- oxidative stress
- bone marrow
- convolutional neural network
- cell proliferation
- fluorescence imaging