Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency.
Eunmi BanHaejin KwonHong Seog SeoYoung Sook YooEun Joo SongPublished in: BMC biotechnology (2021)
This is the first study to optimize extraction and qRT-PCR conditions, while improving miRNA yields and minimizing the loss of extracted miRNA by evaluations of the amplification efficiency.