Temporally distinct roles of Aurora A in polarization of the C. elegans zygote.
Nadia I ManziBailey N de JesusYu ShiDaniel J DickinsonPublished in: bioRxiv : the preprint server for biology (2023)
During asymmetric cell division, coordination of cell polarity and the cell cycle is critical for proper inheritance of cell fate determinants and generation of cellular diversity. In Caenorhabditis elegans ( C. elegans ), polarity is established in the zygote and is governed by evolutionarily conserved Partitioning defective (PAR) proteins that localize to distinct cortical domains. At the time of polarity establishment, anterior and posterior PARs segregate to opposing cortical domains that specify asymmetric cell fates. Timely establishment of these PAR domains requires a cell cycle kinase, Aurora A (AIR-1 in C.elegans ). Aurora A depletion by RNAi causes a spectrum of phenotypes including no posterior domain, reversed polarity, and excess posterior domains. How depletion of a single kinase can cause seemingly opposite phenotypes remains obscure. Using an auxin-inducible degradation system, drug treatments, and high-resolution microscopy, we found that AIR-1 regulates polarity via distinct mechanisms at different times of the cell cycle. During meiosis I, AIR-1 acts to prevent the formation of bipolar domains, while in meiosis II, AIR-1 is necessary to recruit PAR-2 onto the membrane. Together these data clarify the origin of the multiple polarization phenotypes observed in RNAi experiments and reveal multiple roles of AIR-1 in coordinating PAR protein localization with the progression of the cell cycle.
Keyphrases
- cell cycle
- cell proliferation
- high resolution
- single cell
- cell therapy
- emergency department
- stem cells
- dna methylation
- protein kinase
- small molecule
- bipolar disorder
- optical coherence tomography
- gene expression
- genome wide
- deep learning
- mitochondrial dna
- electronic health record
- protein protein
- artificial intelligence
- arabidopsis thaliana