Refinement of an Established Procedure and Its Application for Identification of Hypoxia in Prostate Cancer Xenografts.
Pernille B ElmingThomas R WittenbornMorten BuskBrita Singers SørensenMathilde Borg Houlberg ThomsenTrine StrandgaardLars DyrskjøtSteffen NielsenMichael R HorsmanPublished in: Cancers (2021)
We have developed a combined autoradiographic/laser-guided microdissection method with broad applicability. Using this approach on fresh frozen tumor material, thereby minimizing the degree of RNA degradation, we showed that the 15-gene hypoxia gene classifier developed in HNSCC may be applicable for adenocarcinomas such as prostate cancer.