Refinement of an Established Procedure and Its Application for Identification of Hypoxia in Prostate Cancer Xenografts.

Pernille B ElmingThomas R WittenbornMorten BuskBrita Singers SørensenMathilde Borg Houlberg ThomsenTrine StrandgaardLars DyrskjøtSteffen NielsenMichael R Horsman

Published in: Cancers (2021)

We have developed a combined autoradiographic/laser-guided microdissection method with broad applicability. Using this approach on fresh frozen tumor material, thereby minimizing the degree of RNA degradation, we showed that the 15-gene hypoxia gene classifier developed in HNSCC may be applicable for adenocarcinomas such as prostate cancer.

Keyphrases

prostate cancer
radical prostatectomy
copy number
genome wide
endothelial cells
genome wide identification
minimally invasive
genome wide analysis
dna methylation
high resolution
bioinformatics analysis