Whole blood vs PBMC: compartmental differences in gene expression profiling exemplified in asthma.

Daniel HeChen Xi YangBasak SahinAmrit SinghCasey P ShannonJohn-Paul OliveriaGail M GauvreauScott J Tebbutt

Published in: Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology (2019)

Whole blood RNA preserved in PAXgene tubes is excellent for producing gene expression data with minimal variability and good sensitivity, suggesting its utility in multi-centre studies requiring measurement of blood gene expression.

Keyphrases

gene expression
dna methylation
genome wide
genome wide identification
chronic obstructive pulmonary disease
electronic health record
lung function
copy number
big data
transcription factor
case control
artificial intelligence