Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus.
Cleide Mara Rosa da SilvaRosa Maria Chura-ChambiLennon Ramos PereiraYraima CordeiroLuís Carlos de Souza FerreiraLigia MorgantiPublished in: BMC biotechnology (2018)
The present results represent an innovative non-denaturing protein refolding process by means of the concomitant use of HHP and alkaline pH. Application of the reported method allowed the recovery of ZIKV NS1 at a condition that maintained the antigenic properties of the protein.