Dihydro-Si-rhodamine for live-cell localization microscopy.

Xiaodong ZhangMengmeng ZhangYu YanMingkang WangJin LiYang YuYi XiaoXiao LuoXuhong QianYoujun Yang

Published in: Chemical communications (Cambridge, England) (2021)

Fluorophores with photo-modulatory fluorescence properties are valuable for cutting-edge localization microscopy. The existing probes are either photo-activatable, or photo-switchable, but not both. We report a probe (DH-SiR), a leuco-dye obtained by reduction of Si-rhodamine, with both photo-activatable and photo-switchable fluorescence. The potential for super-resolution microscopy was showcased.

Keyphrases

single molecule
fluorescent probe
living cells
high resolution
high speed
electron transfer
optical coherence tomography
fluorescence imaging
label free
quantum dots