Disposable DNA Amplification Chips with Integrated Low-Cost Heaters.
Henk-Willem VeltkampFernanda Akegawa MonteiroRemco SandersRemco J WiegerinkJoost LöttersPublished in: Micromachines (2020)
Fast point-of-use detection of, for example, early-stage zoonoses, e.g., Q-fever, bovine tuberculosis, or the Covid-19 coronavirus, is beneficial for both humans and animal husbandry as it can save lives and livestock. The latter prevents farmers from going bankrupt after a zoonoses outbreak. This paper describes the development of a fabrication process and the proof-of-principle of a disposable DNA amplification chip with an integrated heater. Based on the analysis of the milling process, metal adhesion studies, and COMSOL MultiPhysics heat transfer simulations, the first batch of chips has been fabricated and successful multiple displacement amplification reactions are performed inside these chips. This research is the first step towards the development of an early-stage zoonoses detection device. Tests with real zoonoses and DNA specific amplification reactions still need to be done.
Keyphrases
- nucleic acid
- early stage
- low cost
- label free
- circulating tumor
- sars cov
- cell free
- single molecule
- coronavirus disease
- loop mediated isothermal amplification
- mycobacterium tuberculosis
- high throughput
- real time pcr
- circulating tumor cells
- molecular dynamics
- emergency department
- respiratory syndrome coronavirus
- mouse model
- heat stress
- staphylococcus aureus
- electronic health record
- anaerobic digestion
- adverse drug
- hepatitis c virus
- drug induced